For Autoradiography we have terms and definitions in 3 topics. The topics are Bioinformatics, Genome and Microbiology.

Autoradiography (Bioinformatics)

A method used to locate radioisotope-labeled materials which have been separated in gels or are present in blots. The location of the radiolabeled material is determined by overlaying the test material with a photographic film that is sensitive to the radioisotope.

Autoradiography (Genome)

A technique that uses X-ray film to visualize radioactively labeled molecules or fragments of molecules; used in analyzing length and number of DNA fragments after they are separated by gel electrophoresis.

Autoradiography (Microbiology)

A process to detect radioactively labeled molecules (which usually have been separated in an SDS-PAGE or agarose gel) based on their ability to create an image on photographic or X-ray film. This process does not result in a linear relationship between the intensity of the signal and the amount of radioactivity unless special steps are taken. There is now increasing use of phosphorimagers and other modern devices to detect and quantitate radioactive molecules which have been separated in gels.

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