For Western Blot we have terms and definitions in 5 topics. The topics are Bioinformatics, Genome, HIV and AIDs, Microbiology and Sex.

Western Blot (Bioinformatics)

Technique in which specific antibodies are used to identify their antigens from a mixture of proteins. Typically, these proteins mixtures are first separated by electrophoresis and then transfered onto nylon sheets by electrotransfer. Radiolabeled or enzyme-linked antibodies are incubated with the sheets and unbound antibodies washed away allowing the position of the bound antibody to be revealed by autoradiography or color which is formed upon addition of a substrate.

Western Blot (Genome)

A technique used to identify and locate proteins based on their ability to bind to specific antibodies.
See also: DNA, Northern blot, protein, RNA, Southern blotting

Western Blot (HIV and AIDs)

A test for detecting the specific ANTIBODIES to HIV in a person's blood. It commonly is used to verify positive ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA tests). A Western Blot test is more reliable than the ELISA, but it is more difficult and more costly to perform. All positive HIV antibody tests should be confirmed with a Western Blot test.

Western Blot (Microbiology)

A technique for analyzing mixtures of proteins to show the presence, size and abundance of one particular type of protein. Similar to Southern or Northern blotting (see "Blotting"), except that (1) a protein mixture is electrophoresed in an acrylamide gel, and (2) the "probe" is an antibody which recognizes the protein of interest, followed by a radioactive secondary probe (such as 125I-protein A).

Western Blot (Sex)

A blood test for HIV antibodies.

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